Attenuation of Staphylococcus Aureus By Pomegranate Peel Extracts

Abstract

Pomegranate Peel extractions used as quorum sensing inhibitor (QSI) against Staphylococcus aureus (which isolated from soft white cheese in local market), different solvents used to obtain different active ingredient groups, minimum inhibitory concentration of each group were determined then sub-inhibitory concentration was used as (QSI). We get that all groups have ability to inhibit quorum sensing in Staphylococcus aureus in different ratio. By using Real-time PCR found that the different Pomegranate Peel extract groups can inhibit the expression of some quorum sensing and virulence factor genes ( Sea, Seb, AgrA, RNAIII, and Hla). Also Pomegranate Peel extracts groups have a role in biofilm reduction in Staphylococcus aureus significantly, furthermore the extract groups significantly affect the growth curve of this bacteria. Our results idicated that the pomegranate peel extracts fight infections by supressing virulence factors, may be due to blocking autoinducer receptors.