Attenuation of Staphylococcus Aureus By Pomegranate Peel Extracts

  • Abdulilah Saleh Ismaeil Biology Department, Science College, Salahaddin University, Erbil, Erbil, Kurdistan Region, Iraq.
  • Fraidoon Abdulqadr Salih Biology Department, Science College, Salahaddin University, Erbil, Erbil, Kurdistan Region, Iraq.
Keywords: Staphylococcus Aureus, Pomegranate Peel Extract, Quorum Sensing Inhibitor, Biofilm, Growth Curve.


Pomegranate Peel extractions used as quorum sensing inhibitor (QSI) against Staphylococcus aureus (which isolated from soft white cheese in local market), different solvents used to obtain different active ingredient groups, minimum inhibitory concentration of each group were determined then sub-inhibitory concentration was used as (QSI). We get that all groups have ability to inhibit quorum sensing in Staphylococcus aureus in different ratio. By using Real-time PCR found that the different Pomegranate Peel extract groups can inhibit the expression of some quorum sensing and virulence factor genes ( Sea, Seb, AgrA, RNAIII, and Hla).

Also Pomegranate Peel extracts groups have a role in biofilm reduction in Staphylococcus aureus significantly, furthermore the extract groups significantly affect the growth curve of this bacteria. Our results idicated that the pomegranate peel extracts fight infections by supressing virulence factors, may be due to blocking autoinducer receptors.     


ALTEMIMI A, LAKHSSASSI N, BAHARLOUEI A, WATSON DG, LIGHTFOOT DA. Phytochemicals: Extraction, Isolation, and Identification of Bioactive Compounds from Plant Extracts. Plants (Basel). 2017;6(4).

AL-ZOREKY N S. Antimicrobial activity of pomegranate (Punica granatum L.) fruit peels.International Journal of Food Microbiology.2009;134,244–248.

ARUN N. & SINGH D. P. Punica granatum: a review on pharmacological and therapeutic properties.International Journal of Pharmaceutical Sciences and Research.2012;3(5),1240–1245.

CELIK,I.,TEMUR,A.,ISIK,I...Hepato protective role and antioxidant capacity of pomegranate (Punicagranatum) flowers infusion against trichloro acetic acid- exposed in rats.Food and Chemical Toxicology: An International Journal Published for the British Industrial Biological Research Association. 2009;47,145–149.

CHAMBERS HF, DELEO FR. Waves of resistance: Staphylococcus aureus in the antibiotic era. Nat Rev Microbiol. 2009;7(9):629-41.

CHEUNG AL, NISHINA KA, TROTONDA MP, TAMBER S. The SarA protein family of Staphylococcus aureus. Int J Biochem Cell Biol. 2008;40(3):355-61.

DA F, YAO L, SU Z, HOU Z, LI Z, XUE X, et al. Antisense locked nucleic acids targeting agrA inhibit quorum sensing and pathogenesis of community-associated methicillin-resistant Staphylococcus aureus. Journal of applied microbiology. 2017;122(1):257-67.

DUNMAN PM, MURPHY E, HANEY S, PALACIOS D, TUCKER-KELLOGG G, WU S, et al. Transcription profiling-based identification of Staphylococcus aureus genes regulated by the agr and/or sarA loci. Journal of bacteriology. 2001;183(24):7341-53.

ELOFF JN. A sensitive and quick microplate method to determine the minimal inhibitory concentration of plant extracts for bacteria. Planta medica. 1998;64(8):711-3.

EL-SHERBINI G M, IBRAHIM K M, EL SHERBINY E T, ABDEL-HADY N M, MORSY T A . Efficacy of Punica granatum extract on in-vitro and in-vivo control of Trichomonas vaginalis. Journal of the Egyptian Society of Parasitology.2010;40, 229–244
FDA. Bacteriological Analytical Manual. 2001.

GEORGE CISAR EA, GEISINGER E, MUIR TW, NOVICK RP. Symmetric signalling within asymmetric dimers of the Staphylococcus aureus receptor histidine kinase AgrC. Mol Microbiol. 2009;74(1):44-57.


HAYAT S, SABRI A N, MCHUGH T D. Chloroform Extract of Turmeric Inhibits Biofilm Formation, Eps Production and Motility in Antibiotic Resistant Bacteria. The Journal of General and Applied Microbiology, 2017;63, 325-338.

HUSAIN F M, AHMAD I, KHAN M S, AHMAD E, TAHSEEN Q, KHAN M S, ALSHABIB N A. Sub-Mics of Mentha Piperita Essential Oil and Menthol Inhibits Ahl Mediated Quorum Sensing and Biofilm of Gram-Negative Bacteria. Frontiers in Microbiology,2015;6, 420.

JAKOBSEN TH, VAN GENNIP M, PHIPPS RK, SHANMUGHAM MS, CHRISTENSEN LD, ALHEDE M, et al. Ajoene, a sulfur-rich molecule from garlic, inhibits genes controlled by quorum sensing. Antimicrobial agents and chemotherapy. 2012;56(5):2314-25.

KAVANAUGH JS, THOENDEL M, HORSWILL AR. A role for type I signal peptidase in Staphylococcus aureus quorum sensing. Mol Microbiol. 2007;65(3):780-98.

LE KY, OTTO M. Quorum-sensing regulation in staphylococci-an overview. Frontiers in microbiology. 2015;6:1174.

MATTHEW THOENDEL JSK, CARALYN E. FLACK, AND ALEXANDER R. HORSWILL. Peptide Signaling in the Staphylococci. Chem Rev. 2011;111:117-51.

MOHABI S, DAVOOD KALANTAR-NEYESTANAKI, MANSOURI S. Inhibition of Quorum Sensing-Controlled Virulence Factor Production in Pseudomonas aeruginosa by Quercus infectoria Gall Extracts. Iranian journal of Microbiology,2017;9 26-32.

MOHAPATRA DP, THAKUR V, BRAR SK. Antibacterial efficacy of raw and processed honey. Biotechnol Res Int. 2011;2011:917505.

MUYIWA AJOKE AKINDOLIRE OOBACNA. Detection of Antibiotic Resistant Staphylococcus aureus from Milk: A Public Health Implication. International Journal of Environmental Research and Public Health. 2015;12.

M. H. ZWIETERING IJ, F. M. ROMBOUTS, AND K. VAN 'T RIET. Modeling of the Bacterial Growth Curve. Applied and environmental microbiology. 1990;56(6):1875-81.

NAZ S, SIDDIQI R, AHMAD S, RASOOL S A, SAYEED S A . Antibacterial activity directed isolation of compounds from Punica granatum. Journal of Food Science.2007;72, M341–345.

O'TOOLE GA. Microtiter dish biofilm formation assay. Journal of visualized experiments : JoVE. 2011(47).

PAI V, CHANU T R, CHAKRABORTY R., RAJU B., LOBO R., BALLAL M ..Evaluation of the antimicrobial activity of Punica granatum peel against the enteric pathogens: an invitro study.Asian Journal of Plant Science and Research1.2011; (2), 57–62.

PALOMBO EA. Traditional Medicinal Plant Extracts and Natural Products with Activity against Oral Bacteria: Potential Application in the Prevention and Treatment of Oral Diseases. Evid Based Complement Alternat Med. 2011;2011:680354.

REUTER K, STEINBACH A, HELMS V. Interfering with Bacterial Quorum Sensing. Perspect Medicin Chem. 2016;8:1-15.

RICHARD P.NOVICK HFR, STEVEN J.PROJAN JK, MOGHAZEH BKAS. Synthesis of staphylococcal virulence factors is controlled by a regulatory RNA molecule. The EMBO Journal. 1993;12(10):3967-75.

SALLY A.& ROSE PBAMFS. Detection of staphylococcal enterotoxins in dairy products by the reversed passive latex agglutination (SET-RPLA) kit International Journal ofFoodMicrobiology. 1989;8:65-72.


Inhibitory effects of active compounds from Punica granatum pericarp on Verocytotoxin by enetrohaemorrhagic Escherichia coli 0157: H7.Journal of Health Science.2005;51,590–596.

YANG Q, SCHEIE AA, BENNECHE T, DEFOIRDT T. Specific Quorum Sensing Disrupting Activity (a QSI) of Thiophenones and Their Therapeutic Potential. Scientific Reports,2015; 5, 18033.

ZHANG H, ZHENG Y, GAO H, XU P, WANG M, LI A, et al. Identification and Characterization of Staphylococcus aureus Strains with an Incomplete Hemolytic Phenotype. Frontiers in cellular and infection microbiology. 2016;6:146.

ZAHIN, M., AQIL,F., AHMAD,I...Broad spectrum antimutagenic activity of antioxidant active fraction of Punica granatum L. pee lextracts.Mutation Research.2010;703,99–107.

ZEHNPFENNIG B, URBATSCH IL, GALLA HJ. Functional reconstitution of human ABCC3 into proteoliposomes reveals a transport mechanism with positive cooperativity. Biochemistry. 2009;48(20):4423-30.
How to Cite
Ismaeil, A., & Salih, F. (2020). Attenuation of Staphylococcus Aureus By Pomegranate Peel Extracts. Journal of University of Raparin, 7(4), 51-61.